82615
Fluka

Buffer solution HPCE pH 8.0 with methylcellulose

BioChemika, for the separation of DNA and other anions

Synonym

TRIS borate pyromellitic acid electrolyte buffer pH 8.0

MDL number

MFCD00217350

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Descriptions

Other Notes

For the separation of DNA restriction fragments without polyacrylamide filled gels. In addition very large DNA fragments (at least 25,000 bp) can be separated with this buffer¹

Preparation Note

prepared under clean room conditions and filtered through a 0.2 μm membrane filter

Properties

product line

BioChemika

shelf life

(limited shelf life, expiry date on the label)

composition

EDTA, 2.5 mM

methylcellulose high viscosity, 0.5%

TRIS borate, 50 mM

fluorescence

λ_ex 200-600 nm; λ_em 300-800 nm (corresponds)

8.0 (25 °C)

conductivity

~0.8 mS/cm at 20 °C

abs.

UV absorption/200-800 nm in accordance

suitability

in accordance for HPCE

References

Cited Reference

1. M. Strege, A. Lagu Anal. Chem. 63, 1233, (1991) abstract

Related Categories

... Buffer Convenience Packaging > Buffer Solutions

... Biological Buffers > Buffers A to Z

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