Ribonuclease A from bovine pancreas 

Protein determined by E1%/280.

• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples.

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The enzyme only hydrolyzes phosphodiester linkages which originate from pyrimidine-3'-phosphates, as well as a large number of 2',3'-cyclo compounds of cytidine and uridine derivatives¹; The return of pancreatic ribonucleases²

1 U corresponds to the amount of enzyme which hydrolyzes the RNA at a rate constant k = 1 at 25°C and pH 5.0 (Kunitz-units); M. Kunitz, J. Biol. Chem. 164, 563 (1946)

BioChemika

4×cryst.

M_r ~13700

mol wt ~13,700

proteases, none detected

wet ice

−20°C

1. H.G. Gassen, H. Witzel Eur. J. Biochem. 1, 36, (1967) abstract

2. S.A. Benner, R.K. Allemann Trends Biochem. Sci. 14, 396, (1989) abstract

Merck 13,8286

3

RF0760000