SYBR^® Green II RNA gel stain 

package with 500 µl

On denaturing agarose/formaldehyde gels and polyacrylamide/urea gels, the sensitivity of SYBR Green II RNA gel stain is reduced, though still superior to that of ethidium bromide. Without any washing or destaining steps, SYBR Green II can detect as little as 1 ng RNA per band in agarose/formaldehyde gel or polyacrylamide/urea gel using 254 nm epi-illumination, and about 4 ng RNA per band using 300 nm trans-illumination. Staining agarose/ formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or subsequent hybridization in Northern blot analysis as long as 0.1%-0.3% SDS is included in prehybridization and hybridization buffers to remove the dye.

SYBR is a registered trademark of Molecular Probes, Inc.

U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.

BioChemika

10000× concentrate in DMSO

absorption1:1000/481 nm, phosphate buffer 0.68±0.05 (pH 7)

−20°C

1

189 °F

87 °C