93286
Fluka

Trizma^® base

BioUltra, for luminescence, ≥99.8% (T)

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Synonym

2-Amino-2-(hydroxymethyl)-1,3-propanediol

THAM

Tris base

Tris(hydroxymethyl)aminomethane

Trometamol

Molecular Formula

NH₂C(CH₂OH)₃

Molecular Weight

121.14

CAS Number

77-86-1

Beilstein Registry Number

741883

MDL number

MFCD00004679

PubChem Substance ID

24889825

EG/EC Number

201-064-4

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Descriptions

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

grade

for luminescence

product line

BioUltra

assay

≥99.8% (T)

total impurities

insoluble matter, passes filter test

ign. residue

≤0.01% (as SO₄)

loss

≤2% loss on drying, 110 °C

10.5-12.0 (25 °C, 4 M in H₂O)

useful pH range

7 - 9

pKa (25 °C)

8.1

219-220 °C/10 mmHg(lit.)

167-172 °C(lit.)

solubility

H₂O: 4 M at 20 °C, clear, colorless

cation traces

Al: ≤5 mg/kg

Ba: ≤5 mg/kg

Bi: ≤5 mg/kg

Ca: ≤10 mg/kg

Cd: ≤5 mg/kg

Co: ≤5 mg/kg

Cr: ≤5 mg/kg

Cu: ≤5 mg/kg

Fe: ≤5 mg/kg

K: ≤50 mg/kg

Li: ≤5 mg/kg

Mg: ≤5 mg/kg

Mn: ≤5 mg/kg

Mo: ≤5 mg/kg

Na: ≤50 mg/kg

Ni: ≤5 mg/kg

Pb: ≤5 mg/kg

Sr: ≤5 mg/kg

Zn: ≤0.5 mg/kg

4 M in H₂O

UV absorption

λ: 260 nm A_max: 0.10

λ: 280 nm A_max: 0.08

suitability

in accordance for luminescence test

References

Reference

Molloy, M.P., et al. Electrophoresis 19, 837, (1998)

Santoni, V., et al. Electrophoresis 21, 1054, (2000)

Merck

Merck 13,9842

Beilstein

Beil. 4,IV,1903

reference

Aldrich MSDS 1, 1815:C / Corp MSDS 1 (2), 3532:A / FT-IR 2 (1), 542:D / FT-IR 1 (1), 347:C / IR-Spectra (3), 203:H / IR-Spectra (3), 203:G / IR-Spectra (2), 183:E / IR-Spectra (2), 183:D / NMR-Reference 2 (1), 304:A / RegBook 1 (1), 371:C / Sax 6, 2558 / Sigma FT-IR 1 (2), 60:D / Sigma FT-IR 1 (2), 61:C / Structure Index 1, 52:C:5 / Vapor Phase 3, 437:C

Safety

Hazard Codes

Risk Statements

36/37/38

Safety Statements

26-36

WGK Germany

RTECS

TY2900000