93349
Fluka

Trizma^® base

BioUltra, ≥99.8% (T)

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Synonym

2-Amino-2-(hydroxymethyl)-1,3-propanediol

THAM

Tris base

Tris(hydroxymethyl)aminomethane

Trometamol

Molecular Formula

NH₂C(CH₂OH)₃

Molecular Weight

121.14

CAS Number

77-86-1

Beilstein Registry Number

741883

MDL number

MFCD00004679

EG/EC Number

201-064-4

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Descriptions

Other Notes

In a high molar concentration for molecular weight determination of peptides and proteins by gel electrophoresis¹; Electrophoresis of proteins on cellulose acetate membranes²; Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis³; Determination of trace amounts of fluorine from a single sodium carbonate fusion of small geological sample masses⁴.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

product line

BioUltra

assay

≥99.8% (T)

total impurities

insoluble matter, passes filter test

ign. residue

≤0.01% (as SO₄)

loss

≤0.5% loss on drying, 110 °C

10.5-12.0 (25 °C, 4 M in H₂O)

useful pH range

7 - 9

pKa (25 °C)

8.1

219-220 °C/10 mmHg(lit.)

167-172 °C(lit.)

168-172 °C

solubility

H₂O: 4 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤5 mg/kg

sulfate (SO₄^2-): ≤5 mg/kg

cation traces

Al: ≤5 mg/kg

As: ≤0.1 mg/kg

Ba: ≤5 mg/kg

Bi: ≤5 mg/kg

Ca: ≤10 mg/kg

Cd: ≤5 mg/kg

Co: ≤5 mg/kg

Cr: ≤5 mg/kg

Cu: ≤5 mg/kg

Fe: ≤5 mg/kg

K: ≤50 mg/kg

Li: ≤5 mg/kg

Mg: ≤5 mg/kg

Mn: ≤5 mg/kg

Mo: ≤5 mg/kg

Na: ≤50 mg/kg

Ni: ≤5 mg/kg

Pb: ≤5 mg/kg

Sr: ≤5 mg/kg

Zn: ≤5 mg/kg

4 M in H₂O

UV absorption

λ: 260 nm A_max: 0.10

λ: 280 nm A_max: 0.08

References

Cited Reference

1. S.P. Fling, D.S. Gregerson Anal. Biochem. 155, 83, (1986) abstract

2. J. Ambler, M. Rodgers Clin. Chem. 26, 1221, (1980) abstract

3. A. Paulus et al. Electrophoresis 11, 702, (1990) abstract

4. A. Hofstetter et al. Analyst 116, 65, (1991)

Reference

Molloy, M.P., et al. Electrophoresis 19, 837, (1998)

Santoni, V., et al. Electrophoresis 21, 1054, (2000)

Merck

Merck 13,9842

Beilstein

Beil. 4,IV,1903

reference

Aldrich MSDS 1, 1815:C / Corp MSDS 1 (2), 3532:A / FT-IR 2 (1), 542:D / FT-IR 1 (1), 347:C / IR-Spectra (3), 203:H / IR-Spectra (3), 203:G / IR-Spectra (2), 183:E / IR-Spectra (2), 183:D / NMR-Reference 2 (1), 304:A / RegBook 1 (1), 371:C / Sax 6, 2558 / Sigma FT-IR 1 (2), 60:D / Sigma FT-IR 1 (2), 61:C / Structure Index 1, 52:C:5 / Vapor Phase 3, 437:C

Safety

Hazard Codes

Risk Statements

36/37/38

Safety Statements

26-36

WGK Germany

RTECS

TY2900000