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96827 
Fluka 

Renaturation Basic Kit for Proteins 

BioChemika  
 
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Price and Availability
Descriptions
Features and Benefits  
Advantages
• Comprehensive, efficient screening
• Stringent purity standards
• Prefabricated reagents, application sheets, and observation sheets
• Low incidence of undesirable solubility effects

 
General description  
The Renaturation Basic Kit for Proteins is a rapid empirical screening method used to determine the best conditions for the renaturation of proteins which have been solubilized from inclusion bodies. It is known that physical parameters, such as pH, redox potential, ionic strength, low-molecular weight additives and temperature, may have an effect on the efficiency of in vitro renaturation processes. Screening with this kit is an easy method to find renaturation conditions in a defined pH range of 6.5 to 8.5, defined redox potential and ionic strength conditions, as well as the effect of one sulfobetaine, namely 3-(1-1-pyridinio)-1-propanesulfonate. Other parameters (e.g., concentration of the solubilized protein, temperature and additional additives) can easily be added to the basic screening conditions of this kit.
 
Other Notes  
All compounds can be ordered separately. Regular pack size is 100 mL, but inquiries for different sizes are welcome. Sceening kit for checking renaturation conditions for proteins1,2,3
 
Packaging  
10 mL per Component
 
Properties
product line 
BioChemika
 
storage temp. 
2-8°C
References
Cited Reference 
1. A. Hofmann et al. Anal. Biochem. 230, 8, (1995) abstract
 
 
2. R. Rudolph, H. Lilie FASEB J. 10, 49, (1996) abstract
 
 
3. G.-Q. Chen, E. Gouaux Proc. Natl. Acad. Sci. USA 94, 3431, (1997) abstract
Safety
Risk Statements 

 
Safety Statements 

Components
Kit component also available separately 
MES pH 6.5; 0.05M – Sodium chloride 0.1M – PPS 1M solution 83047
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.2M solution 82953
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.2M – PPS 1M solution, BioChemika 72905
 
 
MES pH 6.5; 0.05M – DTT 0.001M Solution solution, BioChemika 73021
 
 
MES pH 6.5; 0.05M – DTT 0.001M – PPS 1M solution, BioChemika 71023
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M solution 93038
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M – PPS 1M solution 85312
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M solution 83809
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M – PPS 1M solution, BioChemika 73194
 
 
MES pH 6.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution, BioChemika 72476
 
 
MES pH 6.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M , PPS 1M solution, BioChemika 77809
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution, BioChemika 78569
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution 72914
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution, BioChemika 78582
 
 
HEPES Sodium salt pH 7.5; 0.05M solution 94717
 
 
HEPES Sodium salt pH 7.5; 0.05M – PPS 1M solution 78593
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M solution 87062
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – PPS 1M solution, BioChemika 78579
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M solution 87605
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – PPS 1M solution, BioChemika 72906
 
 
HEPES Sodium salt pH 7.5; 0.05M – DTT 0.001M solution, BioChemika 78152
 
 
HEPES Sodium salt pH 7.5; 0.05M – DTT 0.001M – PPS 1M solution, BioChemika 72824
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M solution, BioChemika 72588
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M – PPS 1M solution, BioChemika 76983
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M solution, BioChemika 73193
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M – PPS 1M solution 80293
 
 
HEPES Sodium salt pH 7.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution 80292
 
 
HEPES Sodium salt pH 7.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution, BioChemika 76951
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution 83068
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution 83796
 
 
HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution 80409
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M solution, BioChemika 75837
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – PPS 1M solution 91810
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M solution 82959
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – PPS 1M solution 83943
 
 
TRIS hydrochloride pH 8.5; 0.05M – DTT 0.001M solution 82305
 
 
TRIS hydrochloride pH 8.5; 0.05M – DTT 0.001M – PPS 1M solution, BioChemika 70603
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M solution, BioChemika 75812
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M – PPS 1M solution 81220
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M solution, BioChemika 72259
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M – PPS 1M solution, BioChemika 72991
 
 
TRIS hydrochloride pH 8.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution, BioChemika 78217
 
 
TRIS hydrochloride pH 8.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution 95739
 
 
TRIS hydrochloride pH 8.5; 0.05M –Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution 82972
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution, BioChemika 72403
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution 85323
 
 
TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution 93039
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.1M solution, BioChemika 72903
 
 
HEPES Sodium salt pH 7.5; 0.05M – 0.1M Sodium chloride, L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution 88503
 
 
MES pH 6.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution 94718
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