A1153
Sigma-Aldrich

Aprotinin from bovine lung

lyophilized powder, 3-8 TIU/mg solid

Synonym

Trypsin inhibitor (basic)

Molecular Formula

C₂₈₄H₄₃₂N₈₄O₇₉S₇

Molecular Weight

6511.44

CAS Number

9087-70-1

MDL number

MFCD00130541

EG/EC Number

232-994-9

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Descriptions

Frequently Asked Questions

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Analysis Note

Another commonly used unit of activity is the KIU (Kallikrein Inhibitor Unit).
1 TIU ≅ 1,300 KIU.

Unit Definition

One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes dissociating at pH >10 or <3. Effective concentration equimolar with protease.

General description

Aprotinin is a protein consisting of 58 amino acids, arranged in a single polypeptide chain that is crosslinked by three disulfide bridges.

Properties

form

lyophilized powder

mol wt

mol wt ~6,500

solubility

H₂O: >10 mg/mL

Gene Information

cow ... PTI(404172)

storage temp.

2-8°C

References

Reference

Deutscher, M.P., Maintaining protein stability. Meth. Enzymol. 182, 83-89, (1990)

Dignam, J.D., Preparation of extracts from higher eukaryotes. Meth. Enzymol. 182, 194-203, (1990)

Gebhard, W., Review: Biochemistry of aprotinin and aprotinin-like inhibitors. Res. Monogr. Cell Tissue Physiol. 12, 375, (1986)

Trautschold, I., et al. Biochem. Pharmacol. 16, 59, (1967)

, Beynon, R.J. and Bond, J.S., ed. Proteolytic Enzymes: A Practical Approach, (1989), 247

Merck

Merck 13,761

Safety

Safety Statements

WGK Germany

RTECS

YN5080000

Related Categories

... Protease Inhibitor Specificity Index > Carboxypeptidase Y

... Protease Inhibitor Specificity Index > Chymotrypsin, α-

... A to C > Chymotrypsin, alpha-

... Plasma & Blood Proteins > Coagulation Proteins and Reagents

... Protease Inhibitor Specificity Index > Elastase, leukocyte

... D to K > Elastase, leukocyte

... Protease Inhibitor Specificity Index > Endoproteinase Lys-C

... Essential Biological Reagents > Enzyme Inhibitors

... Subtilisin > Inhibitors

... Protease Inhibitor Specificity Index > Kallikrein tissue

... D to K > Kallikrein tissue

... Protease Inhibitor Specificity Index > Plasmin

... P to Q > Plasmin

... Protease Inhibitors > Protease Inhibitors

... Miscellaneous Peptides > Protease Inhibitors

... Broad Spectrum Inhibitors of Proteolytic Enzyme Classes > Serine Protease Inhibitors

... R to Z > Tissue Plasminogen Activator

... Protease Inhibitor Specificity Index > Trypsin

... R to Z > Trypsin Inhibitors

A1153 - Enzyme Assay (52kb)

MSDS

Specification Sheet

Certificate of Analysis

Certificate of Origin

More Information