Albumin from bovine serum 

For use in vitamin B₁₂ assays.

Initial fractionation by cold alcohol precipitation

Bovine serum albumin (BSA) makes up approximately 50% of the total protein in serum. Although it may be used as a carrier for hapten immunization in animals other than goats and sheep, it is more commonly used as a carrier for hapten in immunoassays. The small size of BSA, its abundance in serum, and the similarity between BSA and albumins from other species makes it less desirable for antibody production.

Vitamin B₁₂ and B₁₂ binding factor-deficient

lyophilized powder

mol wt ~66 kDa

≤1.0 μg/g binding capacity (vitamin B₁₂)

2-8°C

Trigatti, B.L., , Gerber G.E., A direct role of serum albumin in the cellular uptake of long-chain fatty acids. Biochem. J. 308 (Pt 1), 155, (1995)

Merck 13,8542

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