C2432
Sigma-Aldrich

Chloroform

≥99%, contains amylenes as stabilizer

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Synonym

Methylidyne trichloride

Trichloromethane

Molecular Formula

CHCl₃

Molecular Weight

119.38

CAS Number

67-66-3

Beilstein Registry Number

1731042

MDL number

MFCD00000826

PubChem Substance ID

24892487

EG/EC Number

200-663-8

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Descriptions

Application

Suitable for nucleic acid purification. When used with phenol, the efficiency of protein extraction from crude DNA is improved. Can also be used to remove traces of phenol from aqueous DNA and RNA samples. Suitable for lipid extraction and concentration of hydrophobic membrane proteins.

Properties

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99%

contains

amylenes as stabilizer

refractive index

n20/D 1.445(lit.)

60.5-61.5 °C(lit.)

−63 °C(lit.)

density

1.492 g/mL at 25 °C(lit.)

References

Reference

, Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Plainview, NY , (1989), E.3-E.4

Simoes-Barbosa, A., et al., Solubilization of delipidated macrophage membrane proteins for analysis by two-dimensional electrophoresis. Electrophoresis 21, 641-644, (2000) abstract

Seigneurin-Berny, D., et al., Technical Advance: Differential extraction of hydrophobic proteins from chloroplast envelope membranes: a subcellular-specific proteomic approach to identify rare intrinsic membrane proteins. Plant J. 19, 217-228, (1999) abstract

Merck

Merck 13,2160

Beilstein

Beil. 1,IV,42

Fieser

Fieser 1,29 / Fieser 1,30 / Fieser 1,130 / Fieser 3,140 / Fieser 4,22 / Fieser 5,28 / Fieser 8,92 / Fieser 12,517 / Fieser 14,167 / Fieser 15,84

reference

Aldrich MSDS 1, 405:C / Corp MSDS 1 (1), 786:C / FT-IR 2 (1), 117:D / FT-IR 1 (1), 83:A / FT-NMR 1 (1), 122:A / IR-Spectra (3), 51:G / IR-Spectra (2), 49:E / NMR-Reference 2 (1), 81:C / RegBook 1 (1), 81:D / Sax 6, 722 / Structure Index 1, 9:D:10 / Vapor Phase 3, 116:C

Safety

Hazard Codes

Risk Statements

22-38-40-48/20/22

Safety Statements

36/37

RIDADR

UN1888 6.1/PG 3

WGK Germany

RTECS

FS9100000