D5545
Sigma-Aldrich

DL-Dithiothreitol

SigmaUltra, >99% (titration)

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Synonym

threo-1,4-Dimercapto-2,3-butanediol

Cleland’s reagent

DTT

Molecular Formula

HSCH₂CH(OH)CH(OH)CH₂SH

Molecular Weight

154.25

CAS Number

3483-12-3

Beilstein Registry Number

1719757

MDL number

MFCD00004877

PubChem Substance ID

24893941

EG/EC Number

222-468-7

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Descriptions

Application

An excellent reagent for maintaining SH groups in reduced state;?? quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Properties

grade

SigmaUltra

assay

>99% (titration)

total impurities

Insoluble matter, passes filter test

<0.3% 4,5-dihydroxy-1,2-dithiane

4.0-6.0 (20 °C, 0.1 M in H₂O)

41-44 °C(lit.)

solubility

H₂O: 0.1 M at 20 °C, clear, colorless

anion traces

sulfate (SO₄^2-): <0.005%

cation traces

Al: <0.0005%

As: <0.0001%

Ba: <0.0005%

Bi: <0.0005%

Ca: <0.001%

Cd: <0.0005%

Co: <0.0005%

Cr: <0.0005%

Cu: <0.0005%

Fe: <0.0005%

K: <0.005%

Li: <0.0005%

Mg: <0.0005%

Mn: <0.0005%

Mo: <0.0005%

Na: <0.1%

Ni: <0.0005%

Pb: <0.0005%

Sr: <0.0005%

Zn: <0.0005%

abs.

A0.1M/260, H₂O <0.400

A0.1M/280, H₂O <0.100

storage temp.

2-8°C

References

Reference

Cleland, W.W. Biochemistry 3, 480, (1964) abstract

MacDonald, R.J., Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds. Meth. Enzymol. 152, 219, (1987)

Sears, D.W., In the investigation of incremental and kinetic reduction in proteins. Biochemistry 16, 2031, (1977)

Ruegg, U.T., Cleavage of disulfide bonds in proteins. Meth. Enzymol. 47, 111, (1977)

Bacarese-Hamilton, A., Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Ann. N.Y. Acad. Sci. 448, 571, (1985)

Jabusch, J.R., In protein sequence analysis. Anal. Biochem. 91, 532, (1978)

Merck

Merck 13,3412

Beilstein

Beil. 1,III,2360

reference

Aldrich MSDS 1, 840:C / Corp MSDS 1 (1), 1483:A / FT-IR 2 (1), 417:D / FT-IR 1 (1), 263:C / FT-NMR 1 (1), 424:A / IR-Spectra (3), 155:E / IR-Spectra (2), 139:A / NMR-Reference 2 (1), 230:B / RegBook 1 (1), 283:C / Sigma FT-IR 1 (2), 534:A / Structure Index 1, 38:E:8

Safety

Hazard Codes

Risk Statements

22-36/37/38

Safety Statements

26-36

WGK Germany

RTECS

EK1610000

Flash Point(F)

235.4

Flash Point(C)

113

3-10-16-23

Related Categories

... Biochemicals and Reagents > Antioxidants

... Essential Biological Reagents > Carbohydrates

... Denaturation > Disulfide Bridge Cleavage Reagents

... Carbohydrates > Monosaccharide

... Reduction > Others

... Reduction and Oxidation > Reagents for reduction of proteins

D5545 - Product Information Sheet (79kb)

MSDS

Specification Sheet

Certificate of Analysis

Certificate of Origin

More Information