E1769
Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

SigmaUltra

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Synonym

EDAC

EDC hydrochloride

WSC hydrochloride

N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride

Molecular Formula

C₈H₁₇N₃ � HCl

Molecular Weight

191.70

CAS Number

25952-53-8

Beilstein Registry Number

5764110

MDL number

MFCD00012503

PubChem Substance ID

24894433

EG/EC Number

247-361-2

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Descriptions

Biochem/physiol Actions

Water soluble condensing reagent. It is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. It has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, it is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

Properties

grade

SigmaUltra

total impurities

<0.005% Phosphorus (P)

<0.1% Insoluble matter

ign. residue

<0.1%

110-115 °C(lit.)

solubility

H₂O: 0.5 M at 20 °C, clear, colorless

anion traces

sulfate (SO₄^2-): <0.05%

cation traces

Al: <0.0005%

Ca: <0.0005%

Cu: <0.0005%

Fe: <0.0005%

K: <0.005%

Mg: <0.0005%

NH₄⁺: <0.05%

Na: <0.005%

Pb: <0.001%

Zn: <0.0005%

storage temp.

−20°C

References

Reference

Sheehan, J.C., and Ledis, Total synthesis of a monocyclic peptide lactone antibiotic, etamycin. J. Am. Chem. Soc. 95, 875-879, (1973)

Dhaon, M.K., Synthesis of esters. J. Org. Chem. 47, 1962, (1982)

Drabick, J.J., et al., Covalent polymyxin B conjugate with human immunoglobulin G as an antiendotoxin reagent. Antimicrob. Agents Chemother. 42, 583-588, (1998)

Thomas, J.O., et al., Altered arrangement of the DNA in injection-defective lambda bacteriophage. J. Mol. Biol. 123, 149, (1978)

Lundblad, R.L., et al., Modification of carboxyl groups in proteins, review. Chemical Reagents for Protein Modification 2, 105, (1984)

Safety

Hazard Codes

Risk Statements

37/38-41

Safety Statements

26-36/37/39

WGK Germany

RTECS

FF2200000

3-10-21

Related Categories

... Crosslinking > Heterobifunctional Cross-Linking Reagents

MSDS

Specification Sheet

Certificate of Analysis

More Information