GenElute^™ Minus EtBr Spin Column 

GenElute Minus EtBr Spin Columns are based on the GenElute Agarose Spin Column but incorporate a membrane for the selective removal of ethidium bromide.

For additional information, please see www.sigma-aldrich.com/post-reaction.

• Removes up to 95% of EtBr from DNA
• One-step, 10 minute protocol
• No melting or digestion of agarose required
• Simpler and quicker than most methods
• No ethanol precipitation

The DNA band is excised from an agarose gel and loaded onto the spin column. The membranes, embedded within the column, retain agarose and ethidium bromide while allowing DNA to selectively pass through the column into a centrifuge tube during centrifugation.

Up to 95% of ethidium bromide is removed from DNA with a simple 10 minute procedure. GenElute Minus EtBr Spin Columns can recover DNA fragments from 100 bp to 10 kb with typical recoveries of 30 to 35%. The purified DNA can be used directly for ligation, restriction enzyme digestion, cloning, PCR and related manipulations without ethanol precipitation.

GenElute is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

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