EL4 Cell Line murine 

Cell line was established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. These cells have been reported to produce low levels of type G (Gross) virus antigen (J. Nat. Cancer Inst., 48:265 (1972) as well as H-2b, IL-4, and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to PHA.
Maintain cultures at approx. 3-9 × 10⁵ cells/ml at 37 ^oC and 5 % CO₂. Cell growth after resuscitation is slow. Subculture following log growth phase is verified.
Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS.

dry ice

−196°C

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