87021302
Sigma

COS-7 Cell Line from African green monkey kidney

SV40 transformed

Descriptions

Application

COS-7 is derived from CV-1 simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.

Subculture: Split at 70-80 % confluency, approx. 1:3 to 1:10 (1-3 × 10⁴ cells/cm²). Trypsinize using 0.25 % solution, with or without EDTA, 37 ^oC and 5 % CO_2.

Properties

description

Recommended culture medium: DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).

shipped in

dry ice

storage temp.

−196°C