PC-12 Cell Line from rat 

Recommended culture medium: RPMI-1640 + 2 mM L-Glutamine + 10% FBS

Cells were derived from a transplantable rat adrenal phaeochromosytoma and respond reversibly to nerve growth factor by induction of neuronal phenotype. Cells synthesize and store catacholamine, dopamine, and NPP. Used in neurobiological and neurochemical studies. When cells are grown with collagen - Neuronal fibroblasts; without collagen - spherical clusters.

Cells grow in small clusters but adhere poorly to plastic. The cells do grow satisfactorily in suspension in untreated flasks. Maintain cultures between 2-5x10⁵ cells/ml: 5% CO₂; 37°C. For attachment grow in collagen coated flasks (must be collagen type IV), feed 3 times a week and split cultures 1:3 to 1:6 (i.e. seeding at 2-4x10⁴ cells/cm²) using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Growing cultures will be supplied in suspension. On receipt, incubate the flask overnight without opening and on introduction to a type IV collagen coated flask the cells should attach.

dry ice

−196°C

1

C0543, Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Z380520, CollagenPRO COAT^™ cultureware