THP 1 Cell Line human 

Derived from the peripheral blood of a 1-year-old male with acute monocytic leukemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for alpha-napthhyl butyrate esterase produce lysozymes and are phagocytic (both latex beads and sensitized erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Con A, show increased CO₂ production on phagocytosis, and can be differentiated into macrophage-like cells using for example DMSO.
To subculture, start up initally in 15% serum and 10 mM HEPES. Maintain cultures between 2-9 × 100,000 cells/ml in 5% CO₂ at 37 °C. Cells require glycerol as a cryoprotectant.
Recommended culture medium: RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

dry ice

−196°C

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