Fao Cell Line from rat 

A differentiated cell line derived from H4-11-E-C3 (ECACC catalog number 85061112). A versatile tool for studying regulation of mono-oxygenase activities and analyzing their role in the activation of xenobiotics, e.g. carcinogens, drugs, pesticides.
Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6 (2-4 × 10⁴ cells/cm²) Trypsinize using 0.25% solution, with or without EDTA, 37 ^oC and 5% CO₂.
Recommended culture medium: Coon's Modified Ham's F12 + 2 mM L-Glutamine + 10% FBS or Kaign's Modified Ham's F12 + 2 mM L-Glutamine + 45 mg/L ascorbic acid + 18 mg/L myo-inositol + 10% FBS.

dry ice

−196°C

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