90A1
Sigma

α-Naphthyl Acetate (Non-Specific Esterase)

Select reagents packaged in gelatin capsules.

Synonym

α−Naphthyl Acetate (Non-Specific Esterase)

Descriptions

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.

Properties

shelf life

(Expiry date on the label.)

IVD

for in vitro diagnostic use

storage temp.

2-8°C