α-Naphthyl Acetate (Non-Specific Esterase) Kit 

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.

(Expiry date on the label.)

for in vitro diagnostic use

2-8°C

UN 3316 9/PG 3

alpha-Naphthyl Acetate Solution (91-6) 10 mL

Citrate Solution (91-5) 50 mL

Fast Blue BB Base Solution (91-7) 10 mL

Hematoxylin Solution, Gill No. 3 (kit only) 50 mL

Sodium Nitrite Solution (91-4) 10 mL

TRIZMAL™ 7.6 Concentrate (kit only) 50 mL