HeLa Cell Line human 

HeLa was derived from a cervical carcinoma from a 31-year-old Negro patient. This was the first aneuploid line derived from human tissue maintained in continuous cell culture, and has been identified as a contaminant in many other cell lines. HeLa cells are susceptible to Poliovirus type I and adenovirus type 3 and should be handled under laboratory containment level 2.
Subculture: Split at 70-80% confluency, approx. 1:3 to 1:10 (1-3 × 10⁴ cells/cm²). Trypsinize using 0.25% solution with EDTA, 37 °C and 5% CO₂.
Recommended culture medium: EMEM (EBSS) + 2mM L-glutamine + 1% non-essential amino acids + 10% FBS.

dry ice

−196°C

1