Alcohol Oxidase from Hansenula sp. 

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.

Catalyzes the oxidization of primary alcohols to aldehydes.

Molecular Weight: ~600 kDa
Optimum pH: 8.5
pH Range: 5.5-8.5

K_m values with the following substrates:
Allyl alcohol 33.3 mM
Benzyl alcohol 26.6 mM
Ethanol 16.2 mM
Formaldehyde 2.6 mM
Methanol 2.15 mM
N-Butanol 166 mM
n-Propanol 66 mM

Cofactor: FAD

Inhibitors: 1,4-butynediol (irreversible), propargyl alcohol (irreversible), cyclopropanol, cyclopropanone (suicide substrate), formaldehyde, H₂O₂, hydroxylamine, KBr, KCN, methanol (substrate inhibitor), NaN₃, PCMB, propynal, urea, 4-chloromercuribenzoic acid

vacuum-dried powder

−20°C

3

C5913, 4-Chloromercuribenzoic acid