A0847
Sigma

Adenylyl Cyclase Toxin from Bordetella pertussis

≥70% (SDS-PAGE), recombinant, expressed in Escherichia coli, lyophilized powder, ≥80 units/mg protein

MDL number

MFCD00675046

Descriptions

Biochem/physiol Actions

May be used to affect intracellular cAMP levels in mammalian cells.

Reconstitution

When reconstituted with 50 μL of water, each 50 μg vial will contain 20 mM HEPES buffer, pH 7.5, and 8 M urea.

Unit Definition

One unit will produce 1.0 μmol cAMP from ATP per minute at pH 8.0 at 30 °C

Properties

recombinant

expressed in Escherichia coli

assay

≥70% (SDS-PAGE)

form

lyophilized powder

mol wt

mol wt 177 kDa by amino acid sequence

storage temp.

2-8°C

References

Reference

Hewlett, E. L., et al. J. Biol. Chem. 264, 19379, (1989)

Weiss, A. A., and Hewlett, E. L. Annu. Rev. Microbiol. 40, 661, (1986)

Safety

Hazard Codes

Risk Statements

36/37/38-40

Safety Statements

26-36

WGK Germany

Related Categories

... Cyclic Nucleotide Metabolism > Adenylyl Cyclase Activators

MSDS

Certificate of Analysis

Related Categories

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A0847 (Sigma)