A1086
Sigma

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin

≥97% (HPLC), powder

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Synonym

Ac-DEVD-AMC

Molecular Formula

C₃₀H₃₇N₅O₁₃

Molecular Weight

675.64

CAS Number

169332-61-0

MDL number

MFCD00671411

PubChem Substance ID

24890505

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Descriptions

Biochem/physiol Actions

Fluorogenic substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase.

Properties

assay

≥97% (HPLC)

form

powder

composition

Peptide content, ~65%

color

white

solubility

DMSO: 10 mM

storage temp.

−20°C

References

Reference

Nicholson, D.W., Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376, 37, (1995)

Armstrong, R.C., et al., Activation of the CED3/ICE-related protease CPP32 in cerebellar granule neurons undergoing apoptosis but not necrosis. J. Neurosci. 17, 553-562, (1997)

He, J., et al., Protease activation and cleavage of poly(ADP-ribose) polymerase: an integral part of apoptosis in response to photodynamic treatment. Cancer Res. 58, 940-946, (1998)

Marks, N., Activation of caspase-3 and apoptosis in cerebellar granule cells. J. Neurosci. Res. 52, 334, (1998)

Safety

WGK Germany