Monoclonal Anti-Rat Kappa & Lambda Light Chains−Peroxidase antibody produced in mouse 

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

Lyophilized from a solution containing 1% BSA and 0.01% thimerosal

The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.

purified immunoglobulin

RT-39/RL-6

lyophilized powder

antigen mol wt 25 kDa

vial of 0.5 mL

direct ELISA: 1:40,000

dot blot: 1:4,000

immunoblotting (chemiluminescent): 1:20,000

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

IgG1/IgG2a

2-8°C

3