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Synonym
Monoclonal Anti
Descriptions
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Specificity
Recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal.
Properties
antibody form
purified immunoglobulin
clone
RG-96
form
lyophilized powder
does not react with
human, guinea pig, rat, bovine, goat, sheep, horse, canine, feline, pig, chicken
packaging
vial of 0.5 mL
application(s)
direct ELISA: 1:40,000
dot blot: 1:10,000
immunoblotting (chemiluminescent): 1:160,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
isotype
IgG1
storage temp.
2-8°C
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