A1978
Sigma

Monoclonal Anti-β-Actin antibody produced in mouse

clone AC-15, purified immunoglobulin, buffered aqueous solution

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Synonym

Monoclonal Anti-β-Actin

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Descriptions

Application

The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin's solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Other Notes

To view an Actin antibody selection guide, please click here.

Specificity

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

AC-15

form

buffered aqueous solution

mol wt

antigen mol wt 42 kDa

species reactivity

human, bovine, sheep, pig, rabbit, feline, canine, mouse, rat, guinea pig, chicken, carp, Hirudo medicinalis

does not react with

Dictyostelium discoideum

application(s)

immunoblotting: 0.5-1 μg/mL using cell extract of human foreskin fibroblasts or chicken fibroblasts.

immunocytochemistry: 10-40 μg/mL using human foreskin fibroblasts

immunohistochemistry (frozen sections): suitable

indirect immunofluorescence: suitable

microarray: suitable

isotype

IgG1

Gene Information

mouse ... Actb(11461)

rat ... Actb(81822)

human ... ACTB(60)

shipped in

dry ice

storage temp.

−20°C

References

Reference

North, A.J., et al. J. Cell Sci. 107, 445-455, (1994) abstract

Gimona, M., et al. Cell Motil., 108-116, (1994)

North, A.J., et al., Actin isoform compartments in chicken gizzard smooth muscle cells. J. Cell Sci. 107, 445-455, (1994)

Liao, J., et al., Direct reprobing with anti-β-actin antibody as an internal control for western blotting analysis. Biotechniques 28, 216, (2000)

North, A.J., et al., Calponin is localized in both the contractile apparatus and the cytoskeleton of smooth muscle cells. J. Cell Sci. 107, 437-444, (1994)

Gimona, M., et al., β-actin specific monoclonal antibody. Cell Motil. Cytoskeleton 27, 108-116, (1994)

Safety

WGK Germany