A2066
Sigma

Anti-Actin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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MDL number

MFCD00145889

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Descriptions

Frequently Asked Questions

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Immunogen

The C-terminal actin fragment (C11 peptide, Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe) attached to Multiple Antigen Peptide (MAP) backbone).

Other Notes

To view an Actin antibody selection guide, please click here.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as preservative.

Application

May be used to study actin structure and function and to probe binding sites of actin-binding proteins.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

mol wt

antigen mol wt 42 kDa

species reactivity

wide range, amoeba, slime mold, vertebrates

application(s)

immunoblotting: 1:100 using human or animal tissue extracts

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human or animal tissue sections following enzymatic unmasking

immunohistochemistry (frozen sections): suitable using human or animal tissue sections following enzymatic unmasking

indirect immunofluorescence: suitable using cultured chicken fibroblasts

Gene Information

mouse ... Acta1(11459)

human ... ACTA1(58)

rat ... Acta1(29437)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Kayalar, C., et al., Cleavage of actin by interleukin 1 β-converting enzyme to reverse DNase I inhibition. Proc. Natl. Acad. Sci. USA 93, 2234, (1996) abstract

Safety

WGK Germany