| A2074 Sigma |
Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouseclone RG-16, purified immunoglobulin, lyophilized powder |
Synonym
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
Descriptions
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Specificity
Binds only rabbit Igs. Does not react with reduced rabbit Igs.
Physical form
Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal
General description
The antibody reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM which is sensitive to reduction. No cross-reactivity is observed with human IgG, IgA and IgM.
Properties
antibody form
purified immunoglobulin
clone
RG-16
form
lyophilized powder
does not react with
human, guinea pig, rat, bovine, turkey, chicken, goat, sheep, horse, canine, pig, feline
packaging
vial of 0.5 mL
application(s)
direct ELISA: 1:30,000
dot blot: 1:10,000
immunoblotting (chemiluminescent): 1:160,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
isotype
IgG1
storage temp.
2-8°C
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