A2080
Sigma

Monoclonal Anti-Alkaline Phosphatase, Human Placental−Agarose antibody produced in mouse

clone 8B6, purified immunoglobulin, PBS suspension

Synonym

Anti-Phosphatase, Alkaline, Human Placental

Monoclonal Anti-Alkaline Phosphatase, Human Placental

MDL number

MFCD00162106

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Descriptions

Immunogen

Human epidermoid carcinoma cell line expressing placental alkaline phosphatase.

Preparation Note

Prepared using purified antibody coupled to cyanogen bromide-activated agarose.

Specificity

In SDS gels, the product reacts with both Regan and Nagao isozymes of human placental alkaline phosphatase (hPLAP, 130 kDa, 67/130 kDa). By RIA, the antibody binds to hPLAP with an affinity constant of 5 × 10⁹ LM^-1. It does not react with PLAP-like enzymes.

This immunoadsorbant may be used to purify fusion proteins produced using vectors expressing the hPLAP gene. Suitable for affinity purification and immunoprecipitation procedures of human placental alkaline phosphatase either natural or recombinant.

Physical form

Suspension in 0.1 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

8B6

form

PBS suspension

species reactivity

human

extent of labeling

2 mg antibody per mL bed volume

application(s)

immunoprecipitation: suitable

isotype

IgG2a

Gene Information

human ... ALPP(250)

shipped in

wet ice

storage temp.

2-8°C

References

Reference

Durbin, H., et al., Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations. Int. J. Cancer Suppl., 2, 50, (1988)

Durbin, H., et al., Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments. Int. J. Cancer Suppl., 2, 59, (1988)

Safety

WGK Germany