Albumin from bovine serum 

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Initial fractionation by cold alcohol precipitation
Prepared by a modification of Cohn, using cold ethanol, pH, and low temperature precipitation, followed by additional pH adjustment step prior to final drying.

Remainder mostly globulins.

Bovine serum albumin (BSA) makes up approximately 50% of the total protein in serum. Although it may be used as a carrier for hapten immunization in animals other than goats and sheep, it is more commonly used as a carrier for hapten in immunoassays. The small size of BSA, its abundance in serum, and the similarity between BSA and albumins from other species makes it less desirable for antibody production.

Cohn V fraction

≥96% (agarose gel electrophoresis)

lyophilized powder

mol wt ~66 kDa

6.5-7.5 (1% in 0.15 M sodium chloride)

2-8°C

Cohn, E.J., et al. J. Am. Chem. Soc. 68, 459, (1946)

Trigatti, B.L., , Gerber G.E., A direct role of serum albumin in the cellular uptake of long-chain fatty acids. Biochem. J. 308 (Pt 1), 155, (1995)

Merck 13,8542

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