A2264
Sigma

β-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean)

ammonium sulfate suspension, ~50 units/mg protein

Synonym

β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase

β-N-Acetylhexosaminidase

CAS Number

9012-33-3

Enzyme Commission (EC) Number

3.2.1.52 ( BRENDA | IUBMB )

MDL number

MFCD00081402

EG/EC Number

232-730-2

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Descriptions

Analysis Note

At pH 4.0, p-nitrophenyl β-N-acetylgalactosaminide is hydrolyzed at approximately 50% of the rate of hydrolysis of p-nitrophenyl β-N-acetylglucosaminide at pH 5.0.

Biochem/physiol Actions

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 25 °C.

Physical form

Suspension in 2.5 M (NH₄)₂SO₄, pH 7.0

Properties

form

ammonium sulfate suspension

foreign activity

α-N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase and α-L-fucosidase <0.1%

storage temp.

2-8°C

References

Reference

Li, S.C. and Li, Y.T. J. Biol. Chem. 245, 5153, (1970) abstract

Safety

WGK Germany