A2856
Sigma

Anti-ATG7 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym

Anti-APG7-like

Anti-APG7

Anti-APG7L

Anti-Apoptosis-specific-protein

Anti-Autophagy protein 7-like

Anti-Ubiquitin activating enzyme E1-like protein

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Descriptions

Immunogen

synthetic peptide corresponding to amino acids 38-50 of human ATG7, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse and rat.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

mol wt

antigen mol wt ~75 kDa

species reactivity

human, mouse, rat

application(s)

immunoblotting: 0.5-1 μg/mL using whole extracts of rat PC12 and mouse 3T3 cells

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using biotin/ExtraAvidin^®-Peroxidase staining of heat-retrieved sections of human brain

immunoprecipitation: 1-2 μL using human U87 cell lysate

Gene Information

human ... ATG7(10533)

mouse ... Atg7(74244)

rat ... Atg7(312647)

shipped in

dry ice

storage temp.

−20°C