A3646
Sigma

Aminoacyl-tRNA Synthetase from Escherichia coli

buffered aqueous glycerol solution, ≥2,000 units/mg protein

CAS Number

9028-02-8

MDL number

MFCD00130501

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Descriptions

Analysis Note

Protein determined by biuret.

Other Notes

Mixture of amino acid activating enzymes

EC Ligase sub-class 6.1.1

Quality

Crude

Unit Definition

One unit will activate and attach 1.0 picomole (10^-12 mole) of labeled amino acid to tRNA in 10 min at pH 7.6 at 37 °C (amino acid used: L-arginine).

Physical form

Solution in 50% glycerol containing 10 mM Tris HCl, pH 7.2, 10 mM MgCl₂, 30 mM 2-mercaptoethanol and 10 mM KCl

Properties

form

buffered aqueous glycerol solution

shipped in

wet ice

storage temp.

−20°C

Safety

WGK Germany

Related Categories

... General Metabolic Enzymes > Nucleotide Metabolism

A3646 - Enzyme Assay (14kb)

MSDS

Specification Sheet

Certificate of Analysis

More Information

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A3646 (Sigma)