Monoclonal Anti-β-Amyloid antibody produced in mouse 

synthetic β-amyloid peptide (1-40), conjugated to KLH.

The antibody is useful in immunohistochemistry, immunoblotting,¹ ELISA, and competitive ELISA. Also, this antibody has been used to neutralize Aβ assemblies in brains of transgenic mice expressing a mutant form of amyloid precursor protein,² and for in vivo deep tissue imaging using near-IR optical spectrum.³

The antibody reacts specifically with β-amyloid protein. The epitope recognized by the antibody resides within amino acids 1-12 of the β-amyloid protein. It specifically stains amyloid plaques within the cortex and amyloid deposits in blood vessels using formic acid-treated, formalin-fixed, paraffin-embedded, and Methacarn-fixed sections of human Alzheimer's disease (AD) brain tissue.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

purified immunoglobulin

BAM-10

buffered aqueous solution

human

~1.5 mg/mL

immunoblotting: suitable

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using formic-acid treated tissue sections of human Alzheimer disease brain tissue

indirect ELISA: suitable

IgG1

human ... APP(351)

dry ice

−20°C

1. Tan, J., et al., Role of CD40 ligand in amyloidosis in transgenic Alzheimer's mice. Nat. Neurosci. 5, 1288-1293, (2002)

2. Kotilinek, L.A., et al., Reversible memory loss in a mouse transgenic model of Alzheimer's disease. J. Neurosci. 22, 6331-63335, (2002)

3. Skoch, J., et al., Development of an optical approach for noninvasive imaging of Alzheimer's disease pathology. J. Biomed. Opt. 10, 11007, (2005)

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