Monoclonal Anti-γ-Adaptin antibody produced in mouse 

AP-1 adaptor from bovine brain.

The antibody reacts in immunoblotting with the 104 kDa polypeptide of the Golgi adaptor complex AP-1. It reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from neuroblastoma or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants occurring only in some cell types of brain. The antibody does not recognize the γ-subunit in rodents (rat and mouse). In immuno­fluorescence using MDBK cells, human heart fibroblasts, and African green monkey kidney cells (Vero), the binding of antibody appears to be largely confined to the trans-Golgi network. The antibody has been used for studies on the effects of Brefeldin A, a substance causing rapid redistribution of coat proteins associated with the clathrin-coated vesicles that bud from the trans-Golgi network. The antibody is also useful for the immunoaffinity purification of the Golgi adaptor complex AP-1.

ascites fluid

100/3

15 mM sodium azide

bovine, human, monkey

mouse, rat

electron microscopy: suitable

immunoblotting: 1:100 using bovine brain extract

immunoprecipitation: suitable

IgG2b

human ... GGA1(26088), GGA2(23062), GGA3(23163)

dry ice

−20°C

Ahle, S., et al. EMBO J. 7, 919, (1988) abstract

Robinson, M.S., and Kreis, T.E. Cell 69, 129, (1992) abstract

Lindner, R., and Ungewickell, E. Biochemistry 30, 9097, (1991) abstract

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