Actinomycin D 

An antineoplastic antibiotic that inhibits cell proliferation by forming a stable complex with DNA and blocking the movement of RNA polymerase which interferes with DNA-dependent RNA synthesis. Induces apoptosis. Potent antitumor agent. For cell culture applications, actinomycin D is used as a selection agent and is used in banding techniques to differentiate between different regions of chromosomes.

from Streptomyces sp.

~95% (HPLC)

red, powder

251-253

ethanol, DMSO: soluble

2-8°C

Wadkins, R.M., Actinomycin D binding to single-stranded DNA: sequence specificity and hemi-intercalation model from fluorescence and 1H NMR spectroscopy. J. Mol. Biol. 262, 53, (1996)

Glynn, J.M., et al.,, Apoptosis induced by Actinomycin D, Camptothecin or Aphidicolin can occur in all phases of the cell cycle. Biochem. Soc. Trans. 20, 84S, (1992)

Verma, R.S., Babu, A. Manual of Basic Techniques New York, NY , (1989)

Chen, H., et al., DNA bending and unwinding associated with actinomycin D antibiotics bound to partially overlapping sites on DNA. J. Mol. Biol. 258, 457, (1996)

Cohen, S.M., et al., Identification of chromosomal bands replicating early in the S phase of normal human fibroblasts. Exp. Cell Res. 254, 321, (1998)

Hu, B., et al., Kasabach-Merritt syndrome-associated kaposiform hemangioendothelioma successfully treated with cyclophosphamide, vincristine, and actinimycin D. J. Pediatr. Hematol. Oncol. 20, 567, (1998)

Merck 13,2828

Beil. 27,IV,9642

Aldrich MSDS 1, 34:A / Corp MSDS 1 (1), 62:B / RegBook 1 (2), 2651:C / Sax 6, 134 / Sigma FT-IR 1 (1), 610:D / Structure Index 1, 417:B:6

UN3462 6.1/PG 2

3

AU1575000