Monoclonal Anti-α-Adaptin antibody produced in mouse 

AP-2 adaptor polypeptides from bovine brain.

The antibody reacts with the 105 and 110 kDa α-subunits of adaptor complex AP-2 in bovine and rat brain preparations. It reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from rat pheochromocytoma cultured cells (PC12), neuroblastoma, or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain. The antibody stains the intact α-adaptor, as well as the 37 and 40 kDa fragments, but not the 63-66 kDa group of α-fragments obtained by trypsin cleavage of the α-subunit molecules.

ascites fluid

100/2

15 mM sodium azide

bovine, human, rat

immunoblotting: 1:200 using a crude preparation of synaptic vesicles obtained from rat cerebral cortex

IgG2a

human ... AP2A1(160)

rat ... Ap2a1_predicted(308578)

dry ice

−20°C

Ahle, S., et al. EMBO J. 7, 919, (1988) abstract

Lindner, R., and Ungewickell, E. Biochemistry 30, 9097, (1991) abstract

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