A4418
Sigma

Ammonium sulfate

for molecular biology, ≥99.0%

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Molecular Formula

(NH₄)₂SO₄

Molecular Weight

132.14

CAS Number

7783-20-2

MDL number

MFCD00003391

PubChem Substance ID

24890861

EG/EC Number

231-984-1

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Descriptions

Application

May be used for the precipitation or fractionation of proteins or for purification of antibodies. Useful for crystallographic analysis of nucleic acids and proteins.

Properties

grade

for molecular biology

assay

≥99.0%

total impurities

≤0.005% Insolubles

>280 °C (dec.)(lit.)

density

1.77 g/mL at 25 °C(lit.)

anion traces

chloride (Cl^-): ≤5 ppm

cation traces

Cu: ≤5 ppm

Fe: ≤5 ppm

Zn: ≤5 ppm

heavy metals (as Pb): ≤2 ppm

abs.

A1M/260 ≤0.03

A1M/280 ≤0.025

foreign activity

DNase, RNase, and protease, none detected

References

Reference

Englard, S. and Seifter, S., Precipitation techniques. Meth. Enzymol. 182, 285-300, (1990)

Kent, U.M., Purification of antibodies using ammonium sulfate fractionation or gel filtration. Methods Mol. Biol. 115, 11-18, (1999)

Yaremchuk, A., et al., Improved crystals of Thermus thermophilus prolyl-tRNA synthetase complexed with cognate tRNA obtained by crystallization from precipitate. Acta Crystallogr. D (Biol. Crystallogr.) 56, 197-199, (2000)

Dock, A.C., et al., Crystallization of transfer ribonucleic acids. Biochimie 66, 179-201, (1984)

Merck

Merck 13,559

reference

FT-IR 2 (3), 4751:A / FT-IR 1 (2), 1261:B / IR-Spectra (3), 1521:E / IR-Spectra (3), 762:C / RegBook 1 (3), 3439:D / Sax 6, 268

Safety

WGK Germany

RTECS

BS4500000