Enhanced Avian Reverse Transcriptase [eAMV^™ RT] 

Enhanced Avian (eAMV) Reverse Transcriptase is a highly purified, avian myeloblastosis virus reverse transcriptase (AMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT).
This exceptionally robust AMV-RT has an enhanced ability to detect low abundance messages, transcribe through difficult secondary structure at elevated temperatures (up to 65 °C), and transcribe mRNA templates up to 14.1 kb.

• Greater sensitivity for low abundance mRNA
• Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
• Efficient generation of full-length cDNA, up to 14.1 kb

Provided with a vial of 10× reaction buffer.

One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)_12-18 as a primer.

eAMV is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

20 units/μL

wet ice

−20°C

Brooks, E.M., et al., Secondary structure in the 3′-UTR of EGF and the choice of reverse transcriptases affect the detection of message diversity by RT-PCR,. Biotechniques 19, 806-812, (1995) abstract

Tosh, C., et al., One-tube and one-buffer system of RT-PCR amplification of 1D gene of foot-and-mouth disease virus field isolates. Acta Virol. 41, 153-155, (1997) abstract

Dukas, K.P., et al., Quantitation of changes in the expression of multiple genes by simultaneous polymerase chain reaction,. Anal. Biochem. 215, 66-72, (1993) abstract

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R5277, RT-PCR Protocols