A4541
Sigma

Anti-Biotin−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym

Anti-Biotin

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Descriptions

Immunogen

Biotin-KLH conjugate

Preparation Note

Prepared by the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Specificity

Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal

Application

In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

application(s)

direct ELISA: 1:2,000

shipped in

dry ice

storage temp.

−20°C