ANTI-FLAG^® M1 Agarose Affinity Gel 

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For purification of N-terminal FLAG fusion proteins. Since binding is Ca²⁺-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.

• Typically purify fusion proteins from crude lysates to single band purity in just one chromatography step.
• Fusion protein may be eluted from affinity resin by mild elution with EDTA.
• A solution of FLAG peptide can be used for gentle, non-denaturing elution of FLAG fusion proteins.

To view FLAG^® technology literature, visit www.sigma-aldrich.com/flagliterature.

Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide

ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

FLAG products and/or their use are covered by one or more the following Sigma-Aldrich owned patents: US 4,703,004; US 4,851,351; US 5,011,912; CA 1307752; EP 150126; EP 335899; JP 198150 and JP 2665359.

≥0.6 mg/mL, gel binding capacity

−20°C

1