A5060
Sigma

Anti-Actin (20-33) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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MDL number

MFCD01092261

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Descriptions

Immunogen

N-terminal actin peptide (amino acid residues 20-33) attached to a multiple antigen peptide (MAP) backbone.

Other Notes

To view an Actin antibody selection guide, please click here.

Specificity

The antibody shows a broad reactivity among actin isoforms and across a range of organisms. It specifically stains typical stress fibers in cultured cells using an indirect immunofluorescent labeling technique and can be used as a probe of the N-terminal region of actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

Properties

antibody form

IgG fraction of antiserum

form

buffered aqueous solution

mol wt

antigen mol wt 42 kDa

species reactivity

invertebrates, wide range (animal)

application(s)

immunoblotting: 1:250 using human and animal tissue extracts

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human or animal tissue sections

immunohistochemistry (frozen sections): suitable using human or animal tissue sections

indirect immunofluorescence: 1:200 using cultured human fibroblasts

microarray: suitable

Gene Information

human ... ACTA1(58)

mouse ... Acta1(11459)

rat ... Acta1(29437)

shipped in

dry ice

storage temp.

−20°C

Safety

WGK Germany

NWG