Monoclonal Anti-β-Actin antibody produced in mouse 

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

To view an Actin antibody selection guide, please click here.

Recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin's solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting.

ascites fluid

AC-74

antigen mol wt 42 kDa

15 mM sodium azide

wide range, Drosophila, Hirudo medicinalis, bovine, carp, cat, chicken, canine, guinea pig, human, mouse, pig, rabbit, rat, sheep

immunoblotting: 1:5,000 using cultured human or chicken fibroblast cell extracts

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

indirect ELISA: suitable

indirect immunofluorescence: 1:1,000 using cultured human or chicken fibroblasts

IgG2a

mouse ... Actb(11461)

human ... ACTB(60)

rat ... Actb(81822)

dry ice

−20°C

Gimona, M., et al., β-actin specific monoclonal antibody. Cell Motil. Cytoskeleton 27, 108-116, (1994)

North, A.J., et al., Calponin is localized in both the contractile apparatus and the cytoskeleton of smooth muscle cells. J. Cell Sci. 107, 437-444, (1994)

North, A.J., et al., Actin isoform compartments in chicken gizzard smooth muscle cells. J. Cell Sci. 107, 445-455, (1994)

Liao, J., et al., Direct reprobing with anti-β-actin antibody as an internal control for western blotting analysis. Biotechniques 28, 216, (2000)

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CSAA1, Panorama^® Antibody Microarray - Cell Signaling Kit