A5441
Sigma

Monoclonal Anti-β-Actin antibody produced in mouse

clone AC-15, ascites fluid

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Synonym

Monoclonal Anti-β-Actin

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Descriptions

Frequently Asked Questions

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Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Other Notes

To view an Actin antibody selection guide, please click here.

Specificity

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.

The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin's solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.

Properties

antibody form

ascites fluid

clone

AC-15

mol wt

antigen mol wt 42 kDa

contains

15 mM sodium azide

species reactivity

human, bovine, sheep, pig, rabbit, feline, canine, mouse, rat, guinea pig, chicken, carp, Hirudo medicinalis

does not react with

Dictyostelium discoideum

application(s)

immunoblotting: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

indirect ELISA: suitable

indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts

isotype

IgG1

Gene Information

human ... ACTB(60)

mouse ... Actb(11461)

rat ... Actb(81822)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Gimona, M., et al., β-actin specific monoclonal antibody. Cell Motil. Cytoskeleton 27, 108-116, (1994)

North, A.J., et al., Actin isoform compartments in chicken gizzard smooth muscle cells. J. Cell Sci. 107, 445-455, (1994)

Liao, J., et al., Direct reprobing with anti-β-actin antibody as an internal control for western blotting analysis. Biotechniques 28, 216, (2000)

North, A.J., et al., Calponin is localized in both the contractile apparatus and the cytoskeleton of smooth muscle cells. J. Cell Sci. 107, 437-444, (1994)

Safety

WGK Germany

Related Categories

... Alphabetical Index > A

... Clone Index > A

... Antibodies to Cytoskeleton > Antibodies to Actin Isoforms

A5441 - Datasheet (78kb)

A5441-blot (14kb)

MSDS

Certificate of Analysis

Certificate of Origin

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