| A5463 Sigma |
Anti-Acetylated Proteins antibody produced in rabbit~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution |
Descriptions
Immunogen
synthetic acetylated peptide corresponding to the N-terminus of human histone H4 (amino acids 1-20, with N-ε-acetylated lysines at positions 5,8,12 and 16) conjugated to KLH.
Specificity
Reacts with several acetylated proteins (e.g., acetylated histone H4 [12 kDa], acetylated H1 and H2b and acetylated BSA). Does not recognize acetylated histone H3.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Properties
antibody form
affinity isolated antibody
form
buffered aqueous solution
species reactivity
human, chicken, bovine
concentration
~0.5 mg/mL
application(s)
dot blot: 1:1,000 using Acetylated Histones H4, H1, H2b and acetylated BSA
immunoblotting: 1:1,000 using Whole cell extract of human epitheloid carcinoma HeLa cell line treated with sodium butyrate
indirect immunofluorescence: 1:1,000 using methanol/acetone-fixed chicken fibroblats cells
microarray: suitable
shipped in
dry ice
storage temp.
−20°C
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