A5588
Sigma

Monoclonal Anti-polyHistidine−Alkaline Phosphatase antibody produced in mouse

clone HIS-1, purified immunoglobulin, buffered aqueous glycerol solution

Synonym

Monoclonal Anti-polyHistidine

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Descriptions

Immunogen

recombinant polyHistidine tagged fusion protein.

Specificity

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Properties

antibody form

purified immunoglobulin

clone

HIS-1

form

buffered aqueous glycerol solution

application(s)

immunoblotting: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

Safety

WGK Germany

Related Categories

... Molecular Biology > Antibodies to Peptide and Protein Fusion Tags

... Clone Index > H

... Antibodies and Conjugates > Histidine/Polyhistidine

... Alphabetical Index > PO to PY

... Purification and Detection > Polyhistidine/Histidine Detection

A5588 - Datasheet (72kb)

MSDS

Certificate of Analysis

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A5588 (Sigma)