A5691
Sigma

Monoclonal Anti-Actin, α-Smooth Muscle–Alkaline Phosphatase antibody produced in mouse

clone 1A4, purified immunoglobulin, buffered aqueous glycerol solution

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Synonym

Monoclonal Anti-Actin, α-Smooth Muscle

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Descriptions

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Other Notes

To view an Actin antibody selection guide, please click here.

Specificity

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide as a preservative.

Properties

antibody form

purified immunoglobulin

clone

1A4

form

buffered aqueous glycerol solution

mol wt

antigen mol wt ~42 kDa

species reactivity

human, mouse, rat, bovine, chicken, frog, goat, guinea pig, rabbit, canine, sheep, snake

application(s)

immunoblotting: 1:100 using chicken gizzard extract

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections

isotype

IgG2a

Gene Information

rat ... Acta2(81633)

mouse ... Acta2(11475)

shipped in

wet ice

storage temp.

2-8°C

References

Reference

Skalli, O., et al., A monoclonal antibody against α-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell Biol. 103, 2787, (1986)

Safety

WGK Germany