A6132
Sigma

Apyrase from potato

ATPase 3-40 units/mg protein, lyophilized powder

Synonym

Adenosine 5′-diphosphatase

Adenosine 5′-triphosphatase

CAS Number

9000-95-7

Enzyme Commission (EC) Number

3.6.1.5 ( BRENDA | IUBMB )

MDL number

MFCD00130542

EG/EC Number

232-569-8

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Descriptions

Application

At least two isoenzymes are found in different varieties of S. tuberosum:^1,² one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+P_i → AMP+2P_i.

Other Notes

Mixture of high and low ratio isoenzymes.

Packaging

Sold on the basis of ATPase units.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physical form

Partially purified, lyophilized powder containing sodium acetate buffer salts, pH 5.5.

Enhanced Quality Profile

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Properties

type

Grade I

form

lyophilized powder

composition

Protein, 30-60%

quality classification

Premium

storage temp.

−20°C

References