| A6975 Sigma |
Apoptosis Techniques and Protocols, 2nd ed. |
General description
This edition describes in detail state-of-the-art techniques for studying neuronal cell death. These methods solve a variety of research problems that include the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins in situ. Included are hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays.
Table of Contents
Molecular Characterization of the Proapoptotic Protein Bax
Assessing Cytochrome-c Release from Mitochondria
Monitoring Caspases in Neuronal Cell Death
HSV Amplicon Vectors in Neuronal Apoptosis Studies
Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons
Designing Microarray Experiments for Neurobiology
Analysis of Gene Expression by Nylon Membrane cDNA Arrays
Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs
Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons
Detection and Analysis of Synaptosis
In Situ Detection of Apoptotic Neurons
Apoptotic and Oxidative Indicators in Alzheimer's Disease
Index
Assessing Cytochrome-c Release from Mitochondria
Monitoring Caspases in Neuronal Cell Death
HSV Amplicon Vectors in Neuronal Apoptosis Studies
Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons
Designing Microarray Experiments for Neurobiology
Analysis of Gene Expression by Nylon Membrane cDNA Arrays
Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs
Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons
Detection and Analysis of Synaptosis
In Situ Detection of Apoptotic Neurons
Apoptotic and Oxidative Indicators in Alzheimer's Disease
Index
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