Albumin from bovine serum 

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Initial fractionation by heat shock
Prepared from pasteurized serum, further processed using charcoal filtration and extensive diafiltration to be essentially fatty acid free. Processed to reduce interference of T₃, T₄ and insulin in RIA assays.

Bovine serum albumin (BSA) makes up approximately 50% of the total protein in serum. Although it may be used as a carrier for hapten immunization in animals other than goats and sheep, it is more commonly used as a carrier for hapten in immunoassays. The small size of BSA, its abundance in serum, and the similarity between BSA and albumins from other species makes it less desirable for antibody production.

≥98% (agarose gel electrophoresis)

lyophilized powder

mol wt ~66 kDa

<0.02%

γ-globulin, essentially free

~7 (1% in 0.15 M NaCl)

suitable for diluent in ELISA applications

2-8°C

Trigatti, B.L., , Gerber G.E., A direct role of serum albumin in the cellular uptake of long-chain fatty acids. Biochem. J. 308 (Pt 1), 155, (1995)

Merck 13,8542

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